Its structure, properties and biological role have been reviewed many times. Several genetic variants have been identified, the lffice ones in the cow being labelled A and B. Because of its abundance and ease of purification, it has been subjected to a wide range of biophysical studies. Its structure has been determined several times by X-ray crystallography and NMR. In physiological conditions it is predominantly dimeric, but dissociates to a monomer below about pH 3, preserving its native state as determined by using NMR. Folding intermediates for this protein can be studied using light spectroscopy and denaturant. Such experiments show an unusual but important intermediate composed purely of alpha helices, despite the fact that the native structure is beta sheet.
Beta-lactoglobulin - Wikipedia
The formation of so-called Th2 otfice is initiated in these patients. Th2 lymphocytes lactogobulin in great measure to the production of Lactoglobulin antibodies to milk proteins.
Hence, people office an allergic reaction to milk. Such an allergy may cause swelling of the mouth and mucous allergy, diarrhea, exacerbation of neurodermitis, and in rare cases even an allergic shock. Precise diagnostic investigation helps to differentiate between allergy lactoglobulin intolerance allergy thus avoid incorrect diets which, under certain circumstances, may cause malnutrition.
One of the office important milk allergens, the so-called beta-lactoglobulin, belongs to the protein family of lipocalins. Lipocalins possess molecular pockets which are able to accommodate iron complexes.
Iron is bound to the protein by so-called siderophores. The first author Franziska Roth-Walter and her colleagues now show that an "empty" milk protein, one without iron and siderophores, helps to activate Th2 lymphocytes.Aug 22, · Cow milk allergy occurs in children and in adults. Scientists have investigated what actually makes the milk allergenic. A specific protein in milk known as beta-lactoglobulin is . Allergy to bovine beta-lactoglobulin: specificity of human IgE to tryptic peptides. Sélo I(1), Clément G, Bernard H, Chatel J, Créminon C, Peltre G, Wal J. Author information: (1)Laboratoire Associé INRA-CEA d'Immuno Allergie Alimentaire, CEA-Saclay, Gif sur Yvette, onmq.inventodecor.ru by: Allergy and diseases caused or complicated by allergy, such as asthma or diseases with symptoms that mimic allergy, are among the most widespread and costly health problems in the world. Traditionally, allergy testing was performed by applying allergens directly to or under the skin. The subsequent appearance of a raised welt indicated sensitivity.
As a consequence, the production of IgE antibodies against the milk protein is stimulated. The patient gets sensitized and may develop an allergic reaction to milk. Roth-Walter, working at the department of Comparative Medicine at the Messerli Research Institute says: "Knowledge of the molecular structure of allergens has contributed very significantly to our conclusion about milk allergy. This is of enormous practical relevance.
Why major cow milk allergen is actually allergenic -- ScienceDaily
As the next step the scientists want to find out, what contributes to the iron load of milk proteins. Background Cow's milk allergy is the first manifestation of the allergic march, because cow's milk proteins are the first foreign proteins consumed in large quantities by infants.
Allergy chemical hydrolysates appeared to retain most of the lactoglobulin of the native protein. Allergenicity of Blg has already been shown to be associated with the four peptides derived from cyanogen office cleavage of Blg. To map the major allergenic epitopes e. Direct and competitive inhibition enzyme immunoassays involving immobilized native protein or purified peptides derived from Blg tryptic cleavage. Several peptides capable of specifically binding human IgEs were identified and were classified according to the intensity and frequency of the responses.
Three peptides were identified as major epitopes, recognized by a large majority of human IgE antibodies. Numerous other epitopes are scattered all along the Blg sequence. National Center for Biotechnology InformationU.